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HPLC Analysis of Alkaloids on Discovery® RP-AmideC16

HPLC Analysis of Alkaloids on Discovery® RP-AmideC16


column Discovery RP-AmideC16, 15 cm x 4.6 mm I.D., 5 µm particles (505013)
column temp. 35 °C
mobile phase [A] methanol; [B] 25 mM potassium phosphate, dibasic, pH 3.0; (20:80, A:B)
flow rate 2 mL/min
injection 10 µL
detector UV, 254 nm


Categories Analytical Chromatography, Pharmaceutical Analysis, Alkaloids
Featured Industry Forensics and Toxicology
Pharmaceutical (small molecule)
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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