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HPLC Analysis of Anesthetics, Local on Ascentis® Phenyl

HPLC Analysis of Anesthetics, Local on Ascentis® Phenyl


column Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 µm particles (581616-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium formate (pH 3.0 with formic acid); [B] methanol; (55:45, A:B)
flow rate 1.0 mL/min
sample 50 µg/mL in 85:15, water:methanol
injection 10 µL
detector UV, 254 nm


Categories Analytical Chromatography, Pharmaceutical Analysis, Clinical, anesthetics, Anesthetics, Anesthetic Drugs
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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