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HPLC Analysis of Angiotensins on Discovery® C18

HPLC Analysis of Angiotensins on Discovery® C18


column Discovery C18, 15 cm x 4.6 mm I.D., 5 µm particles (504955)
column temp. 35 °C
mobile phase (A) 5 mM (NH4)H2PO4/NH4OH, pH 7.0; (B) 5 mM (NH4)H2PO4/NH4OH, pH 7.0: CH3CN (50:50, A:B)
gradient 30-60% B in 15 min
flow rate 1 mL/min
detector UV, 220 nm


Categories Analytical Chromatography, Clinical, peptides, Peptides
Featured Industry Clinical
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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