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HPLC Analysis of Antibacterials/Antifungals on Ascentis® RP-Amide, Phosphate Mobile Phase

HPLC Analysis of Antibacterials/Antifungals on Ascentis® RP-Amide, Phosphate Mobile Phase

Conditions

column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 35 °C
mobile phase [A] 10 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid); [B] acetonitrile; (85:15, A:B)
flow rate 1 mL/min
sample 50 µg/mL each in mobile phase
injection 10 µL
detector UV, 254 nm

Description

Categories Analytical Chromatography, Pharmaceutical Analysis, Antibacterials/Antifungals, Antibiotics, Antimicrobials
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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