HPLC Analysis of Artificial Food Colorants in Orange Soft Drink on Chromolith® HighResolution RP-18 endcapped
Conditions
| column | Chromolith HighResolution RP-18, 10 cm x 4.6 mm I.D., 2 µm particles (1.52022.0001) |
| mobile phase | [A] 20mM ammonium acetate pH 6.8 in water; [B] methanol |
| gradient | 5 to 50% B in 6 min; to 100% B in 4 min; held at 100% B for 1 min; to 5% B in 0.01 min |
| flow rate | 1 mL/min |
| pressure | 1526 psi (106 bar) |
| injection | 20 µL |
| detector | UV, 428 nm |
Description
| Categories | Analytical Chromatography, Dyes |
| Featured Industry | Food and Beverages |
| Legal Information | Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes | 5 mL of the beverage was transferred to a 10 mL volumetric flask, sonicated for 5 minutes, then pure water was added to the volume. The solution was filtered by 0.45 μm PTFE syringe filter into a 2mL autosampler vial prior to injection. |
| suitability | application for HPLC |
