Attention:

Certain features of sigmaaldrich.com will be down for maintenance Saturday afternoon/evening, February 24th starting at 3:00 pm CT until 9:00 pm CT.

Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Contents of Energy Drinks With SPE on Chromolith(R) Performance 100-2mm

HPLC Analysis of Contents of Energy Drinks With SPE on Chromolith® Performance 100-2mm

Conditions

sample preparation 1ml Sample on Extrelut N1, wait 10min, eluate with 6ml Ethylacetate, (blow up Ethylacetate with nitrogen, dillute sample in 1ml water )
column Chromolith® Performance, 100 x 2 mm (1.52006.0001)
column temp. 40 °C
mobile phase [A] methanol; [B] 20mM Phosphat buffer pH=5; (10:90, A:B)
gradient 0-3min 190 μl/min, 3,1-12min 425μl/min, 12,1-15min 190μl/min
sample Standard: Ergosterol (Matrix: Dust of a vacuum cleaner)
injection 3 μL
detector 227 nm (cell: 1.4 μL)

Description

Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
Related Links