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HPLC Analysis of Contents of Energy Drinks With SPE on Chromolith(R) Performance 100-2mm

HPLC Analysis of Contents of Energy Drinks With SPE on Chromolith® Performance 100-2mm


sample preparation 1ml Sample on Extrelut N1, wait 10min, eluate with 6ml Ethylacetate, (blow up Ethylacetate with nitrogen, dillute sample in 1ml water )
column Chromolith® Performance, 100 x 2 mm (1.52006.0001)
column temp. 40 °C
mobile phase [A] methanol; [B] 20mM Phosphat buffer pH=5; (10:90, A:B)
gradient 0-3min 190 μl/min, 3,1-12min 425μl/min, 12,1-15min 190μl/min
sample Standard: Ergosterol (Matrix: Dust of a vacuum cleaner)
injection 3 μL
detector 227 nm (cell: 1.4 μL)


Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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