HPLC Analysis of Disaccharides Turanose, Lactulose, and Melibiose on Chromolith® NH2
Conditions
column | Chromolith NH2, 10 cm x 4.6 mm I.D., 2 µm particles (1.52028.0001) |
column temp. | 23 °C |
mobile phase | [A] acetonitrile; [B] water; (85:15, A:B) |
flow rate | 4 mL/min |
pressure | 1102 psi (76 bar) |
sample | turanose 7.7 mg/mL, lactulose 8.0 mg/mL, melibiose 10.6 mg/mL in mobile phase |
injection | 10 µL |
detector | UV, 190 nm |
Description
Categories | Analytical Chromatography, Carbohydrates |
Featured Industry | Food and Beverages |
Legal Information | Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany |
suitability | application for HPLC |
Materials