HPLC Analysis of Disaccharides Turanose, Lactulose, and Melibiose on Chromolith® NH2
Conditions
| column | Chromolith NH2, 10 cm x 4.6 mm I.D., 2 µm particles (1.52028.0001) |
| column temp. | 23 °C |
| mobile phase | [A] acetonitrile; [B] water; (85:15, A:B) |
| flow rate | 4 mL/min |
| pressure | 1102 psi (76 bar) |
| sample | turanose 7.7 mg/mL, lactulose 8.0 mg/mL, melibiose 10.6 mg/mL in mobile phase |
| injection | 10 µL |
| detector | UV, 190 nm |
Description
| Categories | Analytical Chromatography, Carbohydrates |
| Featured Industry | Food and Beverages |
| Legal Information | Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany |
| suitability | application for HPLC |
