HPLC Analysis of E. coli Lysate on Proteomix® SAX-NP3, Demonstration of High Loading Capacity
Conditions
| column | Proteomix SAX-NP3, 5 cm x 4.6mm I.D., 3 µm particles (Z777219) |
| column temp. | 25 °C |
| mobile phase | (A) 20 mM Tris, pH 9.0; (B) A + 0.5 M NaCl |
| gradient | 0 - 100% B in 30 min |
| flow rate | 0.5 mL/min |
| sample | 2.5 mg/mL |
| injection | as indicated in figure |
| detector | UV, 280 nm |
Description
| Categories | Analytical Chromatography, Proteins |
| Featured Industry | Life Science and Biopharma Pharmaceutical (small molecule) |
| Legal Information | Proteomix is a registered trademark of Sepax Technologies |
| suitability | application for HPLC |
