HPLC Analysis of Fluoroacetate and Fluoride on SeQuant® ZIC-HILIC with Radiometric Detection


column Sequant ZIC-HILIC, PEEK, 250 x 4.6 mm, 5 μm, 200 Å (1.50458.0001)
mobile phase [A] 50 mM ammonium hydrogen carbonate; [B] acetonitrile
gradient 10% A for 3 min; to 80% A in 0.5 min; held at 80% A for 5.5 min; to 10% A in 0.5 min
column temp. ambient
flow rate 2 mL/min
detector Bioscan 3000 radiodetector
injection: 50 μL


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Legal Information SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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