HPLC Analysis of Metformin and Related Impurities Cyanoguanidine and Melamine on SeQuant® ZIC-HILIC


column Sequant ZIC-HILIC, PEEK, 250 x 4.6 mm, 5 μm, 200 Å (1.50458.0001)
mobile phase [A] 10 mM ammonium acetate; [B] acetonitrile; (15:85, A:B)
flow rate 1.5 mL/min
pressure 913 psi (63 bar)
column temp. 30 °C ambient
detector 218 nm (cell: 10 μL)
sample 500 ppm metformin and 4 ppm of each impurity in mobile phase
injection: 10 μL


Featured Industry Pharmaceutical (small molecule)
Legal Information SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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