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HPLC Analysis of Oligonucleotides on Proteomix® SAX

HPLC Analysis of Oligonucleotides on Proteomix® SAX


column Proteomix SAX-NP5, 25 cm x 4.6 mm I.D., 5 µm particles (Z777229)
column temp. 25 °C
mobile phase A: 20 mM Tris, pH 8.0 B: A + 0.5 M NaCl
gradient 0 - 100% B in 30 minutes
flow rate 0.5 mL/min
pressure 1073 psi (74 bar)
sample (10 mM each oligonucleotide in water)
injection 5 µL
detector UV 260 nm


Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC


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