HPLC Analysis of Organic Acids in Beverages on Chromolith® HighResolution RP-18 endcapped
Conditions
| sample preparation | sonicate 5 mL of orange drink for 5 minutes, add 0.2 mL of phosphoric acid solution (1M), dilute to 10 mL with water |
| column | Chromolith HighResolution RP-18, 10 cm x 4.6 mm I.D., 2 µm particles (1.52022) |
| column temp. | 30 °C |
| mobile phase | 10 mM diammonium hydrogen phosphate, pH 2.7 |
| flow rate | 1.0 mL/min |
| pressure | 32 bar (464 psi) |
| injection | 20 µL |
| detector | UV, 210 nm |
Description
| Categories | Analytical Chromatography, Organic Acids |
| Featured Industry | Food and Beverages |
| General description | Column Information: 1.52022.0001 |
| Legal Information | Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany |
| suitability | application for HPLC |
Materials
