HPLC Analysis of Organic Acids in Beverages on Chromolith® HighResolution RP-18 endcapped
Conditions
sample preparation | sonicate 5 mL of orange drink for 5 minutes, add 0.2 mL of phosphoric acid solution (1M), dilute to 10 mL with water |
column | Chromolith HighResolution RP-18, 10 cm x 4.6 mm I.D., 2 µm particles (1.52022) |
column temp. | 30 °C |
mobile phase | 10 mM diammonium hydrogen phosphate, pH 2.7 |
flow rate | 1.0 mL/min |
pressure | 32 bar (464 psi) |
injection | 20 µL |
detector | UV, 210 nm |
Description
Categories | Analytical Chromatography, Organic Acids |
Featured Industry | Food and Beverages |
General description | Column Information: 1.52022.0001 |
Legal Information | Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany |
suitability | application for HPLC |
Materials