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HPLC Analysis of Phenolic Antioxidants on Ascentis® C8

HPLC Analysis of Phenolic Antioxidants on Ascentis® C8


column Ascentis C8, 15 cm x 4.6 mm I.D., 5 µm particles (581424-U)
column temp. 30 °C
mobile phase [A] 5% acetic acid; [B] (50:50) methanol:acetonitrile
gradient 30 to 100% B in 10 min; held for 5 min
flow rate 2.0 mL/min
sample as indicated in 50:50 acetonitrile:2-propanol
injection 10 µL
detector UV, 280 nm


Categories Analytical Chromatography, Antioxidants
Featured Industry Cosmetics, Personal Care, and Cleaning Products
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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