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HPLC Analysis of Proteins on BIOshell™ A400 Protein C18

HPLC Analysis of Proteins on BIOshell™ A400 Protein C18


column BIOShell A400 °C18, 10 cm x 2.1 mm I.D., 3.4 µm particles (67463-U)
column temp. 30 °C
mobile phase [A] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 75:25; [B] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 25:75
gradient 20 to 100% B in 8 min
flow rate 0.3 mL/min
pressure 2480 psitotal pressure (193 bar)
sample lysozyme, 0.06; haptoglobulin, 0.06; conalbumin, 0.12; tryspin inhibitor, 0.18; glutamate dehydrogenase, (0.18; ß-galactosidase, 0.12; serum albumin chicken, 0.12 g/L in mobile phase A)
injection 2 µL
detector UV, 215 nm


Analysis Note Analysis of proteins of various hydrophobicity on BIOshell A400 Protein C18 to compare the selectivity and retention times.
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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