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HPLC Analysis of Sildenafil and Impurities on Chromolith(R) Performance (3 mm I.D. columns)

HPLC Analysis of Sildenafil and Impurities on Chromolith® Performance (3 mm I.D. columns)

Conditions

column Chromolith® Performance RP-18 endcapped, 10 cm x 3 mm I.D. (2 columns in series) (1.52001.0001)
column temp. ambient
mobile phase [A] acetonitril; [B] Na-Phosphatbuffer 20mM pH=3,0;
gradient Initial composition: 0-8min 55%A to 45%B isocratic, flow increases from 213μl to 425μl/min, 8,1-20min 65%A to 35%B isocratic, flow 850μl/min
pressure 8913 psi (20-62 bar)
sample Methoxyamin (1mg/ml), Sildenafil Citrat (3mg/ml), Amidin (1mg/ml), Imidon 1 (0.5mg/ml), Imidon 2 (0.5mg/ml), Thiophenethylester (1mg/ml), Acyl-BT-Ester (0,8mg/ml),Aminacid (0,5mg/ml), CHT-Ester (2mg/ml), Acyl-CHT-Ester (2mg/ml),Aminester (0.2mg/ml), Imidochlorid 1 (0.5mg/ml), Imidochlorid 2 (0.3mg/ml)
injection 0.5 μL
detector 254 nm cell: 1.4 μL

Description

Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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