Certain features of will be down for maintenance Saturday afternoon/evening, February 24th starting at 3:00 pm CT until 9:00 pm CT.

Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Sildenafil and Impurities on Chromolith(R) Performance (3 mm I.D. columns)

HPLC Analysis of Sildenafil and Impurities on Chromolith® Performance (3 mm I.D. columns)


column Chromolith® Performance RP-18 endcapped, 10 cm x 3 mm I.D. (2 columns in series) (1.52001.0001)
column temp. ambient
mobile phase [A] acetonitril; [B] Na-Phosphatbuffer 20mM pH=3,0;
gradient Initial composition: 0-8min 55%A to 45%B isocratic, flow increases from 213μl to 425μl/min, 8,1-20min 65%A to 35%B isocratic, flow 850μl/min
pressure 8913 psi (20-62 bar)
sample Methoxyamin (1mg/ml), Sildenafil Citrat (3mg/ml), Amidin (1mg/ml), Imidon 1 (0.5mg/ml), Imidon 2 (0.5mg/ml), Thiophenethylester (1mg/ml), Acyl-BT-Ester (0,8mg/ml),Aminacid (0,5mg/ml), CHT-Ester (2mg/ml), Acyl-CHT-Ester (2mg/ml),Aminester (0.2mg/ml), Imidochlorid 1 (0.5mg/ml), Imidochlorid 2 (0.3mg/ml)
injection 0.5 μL
detector 254 nm cell: 1.4 μL


Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


Related Links