HPLC Analysis of Sulfonamides on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Sulfonamides on Chromolith® High Resolution RP-18e

Conditions

column Chromolith® High Resolution RP-18 encapped, 100 x 4.6 mm (1.52022.0001)
column temp. 40 °C
mobile phase [A] acetonitrile and 20mM ammonium acetate, pH 4.75  (2:98 v/v); [B] acetonitrile and 20mM ammonium acetate, pH4.75  (32:68 v/v);
flow rate 0.7 mL/min
pressure 522 psi (36 bar)
sample Sulfadiazine 1.1 mg/mL, Sulfamethoxazole 0.8 mg/mL, Sulfadimethoxyine, 0.8 mg/mL, Sulfaquinoxaline 1.0 mg/mL, Sulfadimidine 2.6 mg/mL,Sulfamerazine1.0 mg/mL, Sulfathiazole 1.5 mg/mL, Sulfanilamide 0.7 mg/mL, Sulfisoxazole 1.0 mg/mL in diluent (Diluent: Acetonitrile/Water 50/50 v/v )
injection 5 μL
detector 270 nm cell: 11 μL

Description

Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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