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HPLC Analysis of Taxols on Discovery® HS F5

HPLC Analysis of Taxols on Discovery® HS F5


column Discovery HS F5, 15 cm x 2.1 mm I.D., 3 µm particles (567503-U)
column temp. ambient
mobile phase [A] 10 mM ammonium acetate (pH 6.8 unadjusted); [B] acetonitrile
flow rate 0.2 mL/min
sample 10 µg/mL (in 50:50, water:acetonitrile)
injection 10 µL
detector UV, 227 nm


Categories Analytical Chromatography, Antineoplastics
Featured Industry Pharmaceutical (small molecule)
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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