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HPLC Analysis of Taxols on Ascentis® Express F5

HPLC Analysis of Taxols on Ascentis® Express F5


column Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53569-U)
column temp. 30 °C
mobile phase [A] water; [B] acetonitrile; (60:40, A:B)
flow rate 0.3 mL/min
sample 25 mg/L in 70:30, water:methanol
injection 2 µL
detector UV, 227 nm


Analysis Note This application demonstrates the suitability of Ascentis Express F5 for the efficient separation of taxols.
Categories Analytical Chromatography, Antineoplastics
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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