HPLC Analysis of Tetracycline Antibiotics on Ascentis® Phenyl
Conditions
| column | Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 µm particles (581616-U) |
| column temp. | 35 °C |
| mobile phase | [A] 0.1% ammonium acetate; [B] acetonitrile with 0.1% ammonium acetate; (80:20, A:B) |
| flow rate | 1.0 mL/min |
| sample | 50 µg/mL each in 80:20, water:methanol |
| injection | 10 µL |
| detector | UV, 353 nm |
Description
| Categories | Analytical Chromatography, Pharmaceutical Analysis, Antibiotics, Antibiotics, Antimicrobials |
| Featured Industry | Pharmaceutical (small molecule) |
| Legal Information | Ascentis is a registered trademark of Sigma-Aldrich Co. LLC |
| suitability | application for HPLC |
Materials
