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HPLC Analysis of Xanthines on Ascentis® C18

HPLC Analysis of Xanthines on Ascentis® C18

Conditions

column Ascentis C18, 15 cm x 4.6 mm I.D., 5 µm particles (581324-U)
column temp. 35 °C
mobile phase [A] 10 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid); [B] methanol; (94:6, A:B)
flow rate 1 mL/min
sample 50 µg/mL each in mobile phase
injection 10 µL
detector UV, 220 nm

Description

Categories Analytical Chromatography, Bronchodilators
Featured Industry Life Science and Biopharma
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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