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HPLC Analysis of Xanthines on Chromolith(R) NH2 100 - 4.6mm

HPLC Analysis of Xanthines on Chromolith® NH2 100 - 4.6mm

Conditions

column Chromolith NH2, 100 x 4.6 mm (1.52028.0001)
column temp. 23 °C
mobile phase [A] acetonitrile; [B] ammonium acetate buffer 10mM pH=4,5 ; (95:5, A:B)
flow rate 0.5 mL/min
pressure 145 psi 10 Bar
sample Theobromine 0,2mg/ml,Theophylline 0,2mg/ml, Caffein 0,2mg/ml (Diluent: ACN/Water 50/50)
injection 1 μL
detector 270 nm

Description

Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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