LC/MS Analysis of Gamma-Hydroxybutyric Acid (4-Hydroxybutanoic Acid) on SeQuant® ZIC-HILIC


column column: SeQuant ZIC-HILIC, PEEK, 50 x 2.1 mm, 3.5 μm, 200 Å (1.50445.0001)
mobile phase [A] acetonitrile; [B] ammonium acetate; (ratio differs among experiments)
flow rate 0.2 mL/min
pressure 662, 634, and 547 psi (4.6, 4.4, and 3.8 MPa)
detector ESI MS negative ion mode (50 V fragmentor; 2.5 kV cone voltage, SIM ion: 103.2)
injection: 50 μL


Featured Industry Pharmaceutical (small molecule)
Legal Information SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for LC-MS


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