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LC/MS Analysis of Moniliformin on SeQuant® ZIC-HILIC


column Sequant ZIC-HILIC, PEEK, 150 x 4.6 mm, 3.5 μm, 200 Å (1.50449.0001)
mobile phase  [A] acetonitrile; [B] 100 mM ammonium formate
gradient 5 to 15% B in 15 min at 0.5 mL/min; to 50% B in 1 min at 1 mL/min; held at
50% B for 4 min; to 5% B in 1 min; held at 5% B at 0.5 mL/min for 2 min
flow rate 0.5 mL/min
detector diode array at 229 nm, and ESI-MS(-)
injection: 3 μL


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suitability application for LC-MS


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