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LC/MS Analysis of Pain Management Opioids on Ascentis® Express Phenyl-Hexyl

LC/MS Analysis of Pain Management Opioids on Ascentis® Express Phenyl-Hexyl


column Ascentis Express Phenyl-Hexyl 10 cm x 2.1 mm, 2.7 µm particles (53336-U)
column temp. 30 °C
mobile phase [A] water with 0.1% formic acid; [B] methanol with 0.1% formic acid
gradient 10 to 45% B in 3 min; to 100% B in 2 min; held for 2.4 min
flow rate 0.3 mL/min
pressure 6940 psi (478 bar)
sample Pain Management µLti-Component Opiate Mixture-13 (P-071) dilute to 30-300 ng/mL in 99:1, water:methanol
injection 2 µL
detector MS, ESI(+), SIR


Analysis Note Balancing the management of chronic pain against the real possibility of opioid dependence and subsequent abuse is an ongoing conversation in the healthcare industry. Analysts need reliable tools to identify and quantify the drugs and metabolites in patients to tip the balance toward pain managment and away from abuse. LC/MS is becoming the tool of choice for this purpose. Shown here is the rapid, efficient LC/MS analysis of 13 pain manangement opioids on an Ascentis® Express Phenyl-Hexyl U/HPLC column. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Opiate, Analgesics
Featured Industry Forensics and Toxicology
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS


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