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LC/MS Analysis of Peptides, Basic, on Ascentis® C18

LC/MS Analysis of Peptides, Basic, on Ascentis® C18

Conditions

column Ascentis C18, 10 cm x 2.1 mm I.D., 5 µm particles (581326-U)
column temp. 22 °C
mobile phase [A] 20 mM formic acid in water; [B]: 50:50 (v/v) 20 mM formic acid in water:20 mM formic acid in acetonitrile
gradient 20 to 60% B in 20 min
flow rate 0.2 mL/min
injection 2 µL
detector ESI (+), full scan

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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