LC/MS Analysis of Sorbitol and Mannitol on SeQuant ZIC-HILIC

LC/MS Analysis of Sorbitol and Mannitol on SeQuant ZIC-HILIC

Conditions

sample preparation Dissolve 48 mg each of Sorbitol and Mannitol standard in 10 ml volumetric flask and dilute upto the mark with diluent.
column SeQuant ZIC-cHILIC, PEEK, 150 x 4.6 mm, 3 μm, 100 Ã… (1.50661.0001)
column temp. 40 °C (oven), 40 °C (detector cell)
mobile phase [A] acetonitrile; [B] 0.64 g of potassium di-hydrogen phosphate in 1000ml milli-Q water. (Adjust pH 3.0 with ortho-phosphoric acid and filter; (85:15, A:B))
flow rate 0.8 mL/min
pressure 624 psi (43 bar)
sample Dissolve 60 mg of sample in 10 ml volumetric flask (dilute up to the mark with diluent)
injection 10 μL
detector cell: 10 μL (Shimadzu Prominence, R.I.)

Description

Featured Industry Pharmaceutical (small molecule)
suitability application for HPLC

Materials

     
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