UHPLC Analysis of Antihistamines on Ascentis® Express C18
Conditions
| column | Ascentis Express C18, 10 cm x 3.0 mm I.D., 5 µm particles (50526-U) |
| column temp. | 40 °C |
| mobile phase | [A] 0.02M sodium phosphate, pH 2.6; [B] methanol |
| gradient | 50% B for 0.5 min; to 75% B in 2 min; held for 2 minutes |
| flow rate | 1.0 mL/min |
| pressure | 2770 psi (191 bar) |
| sample | Dissolved in 20:80 (v:V0 water:methanol |
| injection | 1.0 µL |
| detector | UV, 230 nm |
Description
| Categories | Analytical Chromatography, Antihistamines |
| Featured Industry | Pharmaceutical (small molecule) |
| Legal Information | Ascentis is a registered trademark of Sigma-Aldrich Co. LLC |
| suitability | application for UHPLC |
Materials
