UHPLC Analysis of Steroids on Ascentis® Express F5

UHPLC Analysis of Steroids on Ascentis® Express F5


column Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.0 µm particles (50863-U)
column temp. 35 °C
mobile phase [A] water; [B] methanol; (50:50, A:B)
flow rate 0.5 mL/min
pressure 11430 psi (788 bar)
sample 50 µg/mL in 85:15, water:methanol
injection 0.5 µL
detector UV, 240 nm


Analysis Note The rapid UHPLC separation of three steroids, a type of lipid derived from cholesterol, on Ascentis Express 2 μm F5 is shown here. The unique pentafluorophenyl stationary phase chemistry of the F5 Column gives it the ability to separate the closely related ring system containing 3-cyclohexanes and 1-cyclopentane in a fused-ring system.
Featured Industry Clinical
Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC


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