UHPLC/MS Analysis of Cannabinoids on Ascentis® Express C18

UHPLC/MS Analysis of Cannabinoids on Ascentis® Express C18


column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 µm particles (50813-U)
column temp. 35 °C
mobile phase [A] 0.1% formic acid; [B] 0.1% formic acid in acetonitrile
gradient 60% B to 100% B in 10 min
flow rate 0.4 mL/min
pressure 7200 psi (496 bar)
sample 100 ng/mL each in methanol
injection 1 µL
detector MS, ESI(+), ESI(-), MRM m/z (see figure for transitions)


Analysis Note Cannabis compounds reportedly have therapeutic efficacy in the treatment of pain, mood disorders, and inflammatory diseases. These standards are used in testing methods by GC/MS, LC/MS, or HPLC for applications in clinical toxicology, testing of cannabis potency or impurity profiling by growers, pharmaceutical research, forensic analysis, and urine drug testing. Shown here is the separation of cannabis compounds on an Ascentis Express C18 column. Highest grade UHPLC solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Featured Industry Food and Beverages
Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC-MS


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