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UHPLC/MS Analysis of Steroids on Titan™ C18

UHPLC/MS Analysis of Steroids on Titan™ C18


column Titan C18, 10 cm x 2.1 mm I.D., 1.9 µm particles (577124-U)
mobile phase (A) water; (B) acetonitrile, both with 0.1% formic acid
gradient 40%B for 1 min, to 90%B in 0.5 min, held for 2 min
flow rate 0.4 mL/min
pressure 4500 psi (310 bar)
injection 2.0 µL
detector MS, ESI(+), MRM


Categories Analytical Chromatography, Steroids
Featured Industry Pharmaceutical (small molecule)
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC


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