UHPLC/MS Analysis of Ten Cannabinoids on Ascentis® Express RP-Amide

UHPLC/MS Analysis of Ten Cannabinoids on Ascentis® Express RP-Amide


column Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.0 µm particles (53913-U)
column temp. 35 °C
mobile phase [A] 0.1% formic acid; [B] 0.1% formic acid in acetonitrile
gradient 60% B to 100% B in 10 min
flow rate 0.4 mL/min
pressure 7600 psi (524 bar)
sample 1000 ng/mL each in methanol
injection 1 µL
detector MS, ESI(+), ESI(-), MRM


Analysis Note Cannabis compounds reportedly have therapeutic efficacy in the treatment of pain, mood disorders, and inflammatory diseases. These standards are used in testing methods by GC/MS, LC/MS, or HPLC for applications in clinical toxicology, testing of cannabis potency or impurity profiling by growers, pharmaceutical research, forensic analysis, and urine drug testing. Shown here is the separation of cannabis compounds on an Ascentis Express RP-Amide column. Highest grade UHPLC solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Herbal medicines, Drugs of Abuse, Pharmaceuticals
Featured Industry Food and Beverages
Forensics and Toxicology
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC-MS


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