TLC Analysis Of Caffeine in Coffee, Coke and Quinine in Tonic Water on TLC Silica gel 60 G F254

TLC Analysis Of Caffeine in Coffee, Coke and Quinine in Tonic Water on TLC Silica gel 60 G F254

Conditions

sample preparation Preparation of coffee in a coffee machine.
Filtration of crude extract with an 0.45 μm syringe filter.
Application by CAMAG ATS4 6 mm bandwise.
plate 1.15161 HPTLC Silica gel 60 RP-18 F 254s MS-grade 20cm x 10 cm (1.151161.0001)
eluent [A] methanol; [B] acetonitrile; (9:1, A:B)
migration distance 6 cm
chamber normal chamber without chamber saturation
sample 0.150 μL
detector UV, 254 nm (Identification by coupling the TLC – MS Interface (CAMAG) with the Advion expression)
(CMS mass spectrometer (+) - ESI mode. Eluent: Acetonitrile / Water 95:5 + 0.1% TFA.) 

Description

suitability application for TLC
Featured Industry Life Science and Biopharma

Materials

     
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