TLC Analysis of Caffeine in Coffee Samples, Quantification of Analyte Concentration

TLC Analysis of Caffeine in Coffee Samples, Quantification of Analyte Concentration

Conditions

sample preparation The boiled coffee samples were extracted and filtered with a 0.45 μm Millex syringe filter.
plate HPTLC Silica gel 60 F254, 20 cm x 10 cm (1.05642.0001)
eluent [A] acetonitrile; [B] methanol; [C] n-Heptane; (6:4:1, A:B:C)
migration distance 5 cm
sample 0.1 μL to 2.0 μL
detector UV, 254 nm

Description

Analysis Note Abstract: HPTLC (High Performance Thin Layer Chromatography) is a very useful analytical technique with very low sample preparation. Shown in the example: the quantification of caffeine in different coffee samples. The boiled coffee samples
were extracted and filtered with a 0.45 μm syringe filter. The plate was pre-conditioned with the mobile phase. After chromatographic separation the plate was scanned with a TLC-Scanner and subsequently quantified.

Results: Caffeine was detected under UV light (254 nm, hRf = 56, Fig. 1), (quantification information Fig. 2). A triple determination of each sample is possible in parallel.

Conclusion: These results demonstrate that caffeine can be easily quantified by TLC with a three-fold determination and minimal sample preparation.

Materials

     
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