SPME-Overcoated Fiber: Physically Robust and Resistant to Fouling

High background samples, such as many foods, pose a challenge to immersion SPME due to the presence of fats, sugars, pigments, and other macromolecules. These can stick to the SPME fiber and reduce its usable life and/or be transferred to the GC, where they may interfere with chromatographic analysis. A new development in SPME fiber technology incorporates a protective overcoating on the fiber. This overcoating, which consists of polydimethylsiloxane (PDMS) protects the fiber, making it more physically robust and less prone to chemical fouling.

 

Overcoated SPME Fiber (PDMS/DVB)

Overcoated SPME Fiber (PDMS/DVB)

 

Appearance of Overcoated  and Non-Overcoated PDMS/DVB SPME Fibers After Repeated Immersion Extractions of Spaghetti Sauce

Appearance of Overcoated and Non-Overcoated PDMS/DVB SPME Fibers
After Repeated Immersion Extractions of Spaghetti Sauce

 

Pesticide Response from Spiked Spaghetti Sauce Extracted by Immersion SPME; Overcoated and Non-Overcoated PDMS/DVB Fibers. Using the Non-Overcoated (non OC) Fiber, a Complete Loss in Response Occurred After 19 Extractions Due to GC System Contamination

Pesticide Response from Spiked Spaghetti Sauce Extracted by Immersion SPME; Overcoated
and Non-Overcoated PDMS/DVB Fibers. Using the Non-Overcoated (non OC) Fiber, a Complete
Loss in Response Occurred After 19 Extractions Due to GC System Contamination

 

Materials

     

References

  1. Matrix compatible solid phase microextraction coating, a greener approach to sample preparation in vegetable matrices. Attilio Naccarato and Janusz Pawliszyn. Food Chemistry, 206, 67-73 (2016)
  2. Souza Silva, É. A.; Pawliszyn, J. Anal. Chem. 2012, 84, 6933–6938.
  3. Souza-Silva, É. A.; Lopez-Avila, V.; Pawliszyn, J. J. Chromatogr. A 2013, 1313, 139–146.
  4. Souza-Silva, É. A.; Pawliszyn, J. J. Agric. Food Chem. 2015, 63, 4464–4477.
  5. Gionfriddo, E.; Souza-Silva, E. A.; Pawliszyn, J. Anal. Chem. 2015, 87, 8448–8456.
  6. Souza-Silva, É. A.; Gionfriddo, E.; Shirey, R.; Sidisky, L.; Pawliszyn, J. Anal. Chim. Acta 2016, 920, 54–62.
  7. Fontanive, F. C.; Souza-Silva, É. A.; Caramao, E.; Zini, C. A.; Pawliszyn, J.; Sidisky, L. M.; Buchanan, M. D.; Vitkuske, D. Reporter, Appl. Newsl. 2016, 34.1, 3–7
  8. Stenerson, K.; Young, T. Reporter, Appl. Newsl. 2016, 34.3, 15–19.
  9. Gionfriddo, E.; Passarini, A.; Pawliszyn, J. J. Chromatogr. A 2016, 1457, 22–28.
  10. Souza-Silva, É. A.; Gionfriddo, E.; Alam, M. N.; Pawliszyn, J. Anal. Chem. 2017, 89, 2978–2985.
  11. De Grazia, S.; Gionfriddo, E.; Pawliszyn, J. Talanta 2017, 167, 754–760.