Oligonucleotide Quality Control of Custom Formats

Delivery of oligonucleotides in custom formats, such as 96- and 384-well plates, presents challenges to ensure consistent quality. Additional quality control measures are in place to verify accuracy for custom formats. These measures may include:

  • Plate orientation and well position verification by MS
  • Proper mixing of primers, e.g. forward and reverse, in a single well, verified by MS (Figure 1)
  • Verification for machine readable barcodes (1D and 2D)
  • Visual inspection of all master and daughter plates

Mixed forward and reverse primers detected by MS

Figure 1. Mixed forward and reverse primers detected by MS.

Conclusion

Additional quality control measures are in place to assess oligonucleotides that are in custom formats. If additional help is needed, please consult our technical services group at oligotechserv@sial.com.