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Fluorous Separation Media

Aldrich ChemFiles 2007, 7.3, 10.

Aldrich ChemFiles 2007, 7.3, 10.

The separation of fluorous compounds by F-SPE (Fluorous Solid Phase Extraction) is a reliable and generic procedure that more greatly resembles filtration than chromatography. The quick separation depends primarily on the presence or absence of a light fluorous tag, not polarity or other molecular features that control traditional chromatography.1 FluoroFlash® silica selectively retains fluorous molecules while non-fluorous compounds are not retained. The fluorous compounds can then be recovered easily through a simple solvent change. FluoroFlash® SPE cartridges are pre-packed in a variety of formats with a proprietary fluorous silica gel.

In Figure 1, a mixture of a non-fluorous dye (blue) and a fluorous dye (orange) are loaded on a fluorous adsorbent (left-hand test tube). The non-fluorous dye can be washed down with a fluorophobic solvent mixture like aqueous methanol (middle test tube). The fluorous dye remains on the adsorbent until the elution with a fluorophilic wash (e.g. with pure methanol, right-hand test tube). The colored dyes of this example were used to visualize the easy separation of a fluorous-tagged product from non-fluorous byproducts with F-SPE (Fluorous Solid Phase Extraction).

Figure 1

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  1. Zhang, W.; Curran, D.P. Tetrahedron 2006, 62, 11837. FluoroFlash® is a registered trademark of Fluorous Technologies, Inc.

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