Alternate Selectivity in HPLC 3: Method Development w/Polar Columns

This 43-minute Supelco web seminar will describe the chemical structure of various types of bonded phases. Some possible explanations why polar RP phases have different selectivity from nonpolar straight-chain alkyl phases will be proposed, and classes of compounds that are best separated by polar RP phases will be shown.

HPLC columns with nonpolar alkyl functional groups, such as C18 and C8, continue to be the most popular choice for reversed-phase method development; however, columns having alkyl chains with an embedded polar group (EPG) and alkyl chains linked to a phenyl group are becoming increasingly important.

Seminar Abstract
The web seminar covers the following points:

  • Introduction to HPLC stationary phases
  • Polar phase alternatives to C18 and C8
  • Method development with polar HPLC columns
  • Embedded Polar Group (EPG) phases
  • Phenyl polar phases
  • Fluorinated and other alternate selectivity phases
  • Aqueous Normal Phase (ANP) or HILIC mode
  • Selectivity Comparisons, using various examples

Presenter Profile
Richard Henry received his B.S. degree in Chemistry from Juniata College in 1963 and Ph.D. in Analytical Chemistry from The Pennsylvania State University in 1966. After a postdoctoral year in separations at Purdue University with Professor L. B. Rogers, he joined DuPont in 1967 at the Experimental Station in Wilmington, DE and became one of the first employees of the DuPont Instrument Products Division in 1969. Dick worked closely with Dr. Jack Kirkland and others in the development of HPLC columns and packing materials while at DuPont. After about 10 years with Spectra-Physics and consulting on the west coast, he joined the Penn State University chemistry faculty in 1985 where he was Director of Analytical Laboratories and taught Instrumental Analysis to chemistry majors. In 1985, he also founded Keystone Scientific, Inc. where he developed HPLC columns and related separation technology. He retired from both Penn State University and Keystone Scientific in 2002, and remains active teaching short courses on separation technology and as a consultant. Dick has research interests in separation mechanisms and all applications of new LC column technology. He is also interested in new analytical techniques, especially the rapidly growing fields of UHPLC, chiral HPLC, LC-MS, and multidimensional separation methods. He served two terms as Chairman of the ACS Subdivision on Separations (1998-2002) and has also served for many years on its Executive Committee.