Webinar: Applying Best in Class Size Exclusion Chromatography Technologies to the Analysis of Antibodies and Glycoproteins

Presented by Judith Vajda and Kevin Ray

60 minutes

Learn about the use of versatile analytical techniques including oligosaccharide analysis, monoclonal antibody aggregate quantification and application of SEC-MS towards high-throughput intact mass glycoprofiling.

High performance liquid chromatography is a fundamental method for research and quality control of biopharmaceutical products.

This webinar focuses on the versatile use of size exclusion chromatography in analysing Antibodies and Glycoproteins. We cover tips & tricks with regards to buffer choice for size exclusion chromatography coupled to mass spectrometry, advantages of fluorescence detection in hydrophobic interaction chromatography as well as the use of buffer additives in size exclusion chromatography of monoclonal antibodies.

The second part of this webinar demonstrates the application of SEC-MS towards high-throughput intact mass glycoprofiling of biotherapeutic antibodies and Fc-fusion proteins.




Judith Vajda is a Senior Lab Specialist at Tosoh Bioscience GmbH. As a member of the technical support team, her work also includes application development. Judith has been involved in purification and analysis of biologics for years. She has a keen interest in applying new technologies for the chromatographic separation of immunoglobulins and related targets. She has presented at several conferences, such as PREP, ISPPP, HIC/RPC, and the BioInnovation Leaders Summit. Judith holds a major in immunology and a diploma degree in technical biology from the University of Stuttgart.

Kevin Ray received his Ph.D. in Analytical Chemistry in 1993 from Texas A&M University. He joined Sigma-Aldrich in 1994 as a Senior Scientist to provide oversight of analytical services and consultation to research staff towards the development of powdered and liquid cell culture media. In 2002 he was appointed Research Manager with responsibilities for the development of nucleic acid purification products and technologies. Since 2005 he has held the position of Manager of Analytical Research & Development, leading a team responsible for providing advanced analytical support to product development groups in Biotech R&D and serving as the corporate center of excellence for mass spectrometry. Dr. Ray has expertise in mass spectrometry based protein assays and protein characterization.