Webinars



Webinar: Genome Engineering to Synthetic Genomics

30 Year Research Odyssey of an Indian Born American Scientist

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 Abstract

Over the 30 years of my scientific research career at the Johns Hopkins Medical Institutions, I have been very fortunate to work on two exciting areas in life sciences: 1) genome engineering (aka genome editing) and 2) synthetic biology (more specifically synthetic genomics). Our basic research on FokI restriction enzyme, led to the creation of zinc finger nucleases (ZFNs), which were the first truly targetable reagents that showed pre-determined DNA sequences could be addressed for cleavage by protein engineering [1-3]. Our studies on the mechanism of cleavage by 3-finger ZFNs established that the preferred substrates were paired binding sites, which doubled the size of the target sequence recognition from 9 to 18 bp, long enough to specify a unique genomic locus in plant and mammalian cells [4]. Soon afterwards, we showed that a ZFN-induced targeted DSB stimulates homologous recombination in cells, using frog oocytes as a model system [5]. Thus, ZFNs ushered in the era of genome editing.

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Speaker Bio
Srinivasan Chandrasegaran
Professor
Department of Environmental Health and Engineering
Johns Hopkins School of Public Health
Baltimore, Maryland 21205, USA
Srinivasan Chandrasegaran Ph.D. is a tenured Professor in the Department of Environmental Health and Engineering at the Johns Hopkins School of Public Health (JHSPH). His lab reported the creation of designer zinc finger nucleases (ZFNs) in 1996. He then showed in 2001 the utility of ZFNs in stimulating site-specific recombination in frog oocytes in a collaboration with Dana Carroll lab. His lab was the first to report the total synthesis of a functional designer eukaryotic (yeast) chromosome, synIII, in 2014.

Webinar: Journey of Protein from Extraction to Purification

30 October 2017
 

 Abstract

Various methods for the extraction and purification of recombinant proteins from bacteria are in use and have been well described. Common methods for cell lysis involve mechanical disaggregation of cells, such as sonication, but this can be time-consuming when working with multiple samples and more importantly can result in localized heating leading to loss of protein activity. In this seminar, we provide data showing that gentle detergent based lysis leads to greater protein activity and higher yields when compared to mechanical methods such as sonication. We also investigate the use of dual purification strategies and explore optimizing conditions to improve the purity of protein purification techniques.

 

Speaker Bio
Dr. Natasha L. Pirman
Application Scientist for Molecular Workflow Tools
Dr. Natasha L. Pirman is an Application Scientist for Molecular Workflow Tools. She is involved in developing new sample preparation products and applications for both protein and nucleic acid applications. Prior in her career she uilized her expertise in protein biochemistry to explore structural protein changes to Intrinsically Disordered Proteins and human kinase disease models for drug target investigations.


 

Webinar: Trusted Tools and Innovation in General and Specialty Cell Culture

15 November 2017
 

 Abstract

Cell culturing represents a fundamental research tool for life scientists by offering a unique ability to grow, manipulate, and analyze populations of cells.  From experiments utilizing the latest gene editing technologies to simple growth assays, high quality tools to ensure reproducible and reliable cell culturing is critical for the success of any life science projects. In this webinar, key considerations for choosing the right tools for various aspects of cell culturing will be presented. Starting from sterile filtration and cell enumeration through micro-porous membrane based functional assays and live cell imaging, audiences will be able to gain insights into parameters affecting cell culturing such as the choice of membranes and pore sizes and also learn about innovative microfluidics based tools for cell enumeration and culturing.

 

Speaker Bio
Jun Park, Ph. D.
Senior Applications Scientist
Jun Park, Ph. D. is Senior Applications Scientist. He studied Biomaterials and Immunology at University of Toronto and did his postdoctoral work at Harvard Medical School investigating small molecule regulators of beta integrins. Since 2006 his primary works have focused on the novel detection technologies, Western blotting, and cell based assays.      

Webinar: Not All Spots are Created Equal: Maximizing ELISpot Data Quality through Workflow Optimization

12 December 2017
 

 Abstract

Precise regulation of lymphocyte function is critical to mounting a specific immune response; this complex process requires exquisite control of processes regulating expression of effector capabilities. Understanding such mechanisms is essential for the development of therapeutics that enhance endogenous immune function. While no one assay can measure all parameters, the ELISpot offers quantitative assessment of effector function(s) at the single cell level with superior sensitivity. We have significantly improved upon ELISpot user-success rates through advances in plate/membrane technologies with our partners, including fluorescent applications, as well as optimized protocols to meet the common pitfalls of this assay. This webinar will focus on two key aspects of the assay that can greatly impact overall performance: choice of plate configuration and the role of apoptotic cells.

 

Speaker Bio
Dr. Amedeo Cappione
Senior Scientist
Technology Development Group
Dr. Amedeo Cappione is a Senior Scientist in the Technology Development Group with an emphasis on Cell Culture Workflow Tools and Applications. In this capacity, he works with Business Development and Marketing partners to identify and assess cutting-edge technological opportunities for collaborative development, licensing, or acquisition. Dr. Cappione has over 20 years of research and product development experience spanning all aspects of Cell and Molecular Biology with a specific focus in cell-based assays.