Measuring Ethanol in Kombucha Tea by HS-SPME: A Walk Through Method Development & Validation

Presenter: Katherine Stenerson

Presenter: Katherine Stenerson

Kombucha, A fermented tea beverage produced by the addition of a mixture of yeast and acetic acid bacteria to a solution of sugar and tea, is an effervescent solution containing phenolics, water soluble vitamins, organic acids and some ethanol. Ethanol testing in kombucha is important to ensure the alcohol content is <0.5% in the United States and avoid being regulated as an alcoholic beverage. Listen to our 15-minute talk to learn about developing an SPME (Solid Phase MicroExtraction) method for alcohol in Kombucha, the method validation and testing of commercial samples.

Katherine Stenerson is an Analytical Sciences Liaison within the Applied Solutions Group at the life science business of Merck KGaA, Darmstadt, Germany, which operates as MilliporeSigma in the U.S. and Canada. Her technical experience and expertise are in developing workflow solutions for extraction and analysis to address problems related to analytical testing, with a focus on the areas of food & beverage, cannabis, and environmental testing. Prior to joining MilliporeSigma, Katherine worked as an analytical chemist in the personal care products industry, and as a GC chemist and supervisor in the environmental testing industry. She has been a member of the American Chemical Society, Chromatography Forum of Delaware Valley and AOAC. Her activities within AOAC include serving on several working groups and an expert review panel.
 

 

Product description Cat. No. Pack size
Supelcowax®10, 30 m x 0.25 mm I.D., 0.50µm 24284 1
SPME fiber assembly, 100µm PDMS for autosampler, 23 ga 57341-U 3
Inlet Liner, Direct (SPME) Type, 0.75 mm I.D. for Agilent 2637501 1
Headspace vial with screw top, clear, 10 mL SU860099 100
Magnetic screw cap for headspace vial w/1.5 mm PTFE Silicone septa SU860103 100
Ethanol, 200 proof anhydrous, >99.5% purity 459836 100ML
Ethanol-d6, >99.5% atom %D 186414 5G
Sodium phosphate, monobasic, for HPLC, 99-101% 52074 50G
Sodium phosphate, dibasic, for HPLC, ACS grade SX0723 500G
Sodium chloride, Emsure, 1 kg 1064041000 1KG