[Skip to Content](https://www.sigmaaldrich.com#main-content) [![MilliporeSigma](https://www.sigmaaldrich.com/static/logos/purple/millipore_sigma.svg)](https://www.sigmaaldrich.com/US/en) Products Cart0 USEN Products [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/US/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/US/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/US/en)[Cloning & Expression](https://www.sigmaaldrich.com/US/en/applications/genomics/cloning-and-expression)x-tracta Gel Extraction Tool # x-tracta Gel Extraction Tool The [x-tracta gel extraction tool](https://www.sigmaaldrich.com/US/en/product/SIGMA/Z722390) ([Z722390](https://www.sigmaaldrich.com/US/en/product/SIGMA/Z722390)) is an easy to use, quick alternative to using scalpel or razor blades for the extraction of DNA and RNA bands from agarose gels following gel electrophoresis. The cutting blade and ergonomic design mean that extractions can be performed in a rapid, easy to follow process with greater accuracy and precision. Simply stated, the superior cutting edge of x-tracta along with its easy, one-handed operation means that it performs better than any other gel extraction solution currently available. ### Benefits of the x-tracta Gel Extraction Tool: - Fast - Easy to Use - One-Handed Procedure - Precise Extraction - High Yield Recovery - Low Failure Rate - Disposable - Safe ## Gel Extraction Procedure: ![xtracta-stepone.jpg](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/protocols/genomics/cloning-and-expression/xtracta-stepone.jpg "xtracta-stepone.jpg") [Step 1:](#) x-tracta gel extraction tool ![xtracta-steptwo.jpg](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/protocols/genomics/cloning-and-expression/xtracta-steptwo.jpg "xtracta-steptwo.jpg") [Step 2:](#) Place tool over desired gel band ![xtracta-stepthree.jpg](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/protocols/genomics/cloning-and-expression/xtracta-stepthree.jpg "xtracta-stepthree.jpg") [Step 3:](#) Press the tool into the agarose gel, gently rock the tool side to side, to extract the desired gel band ![xtracta-stepfour.jpg](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/protocols/genomics/cloning-and-expression/xtracta-stepfour.jpg "xtracta-stepfour.jpg") [Step 4:](#) Expel extracted gel band from tool by a quick snap-action squeeze ## DNA Purification after Band Excision: After excising the desired gel band using the x-tracta tool, we recommend using the __[GenElute™ Gel Extraction Kit](https://www.sigmaaldrich.com/US/en/product/SIGMA/NA1111) (__[NA1111](https://www.sigmaaldrich.com/US/en/product/SIGMA/NA1111)) to bind and elute the DNA. __[GenElute™ Gel Extraction Kit](https://www.sigmaaldrich.com/US/en/product/SIGMA/NA1111) ([NA1111](https://www.sigmaaldrich.com/US/en/product/SIGMA/NA1111))__ The GenElute™ Gel Extraction Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels, or polyacrylamide gels. Typical recovery of DNA is up to 80%. Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice. The isolated DNA is suitable for a variety of downstream applications, such as automated DNA sequencing, PCR, restriction digestion, cloning, and labeling. ## Materials Sorry, an unexpected error has occurred Response not successful: Received status code 500 __Related Protocols__ - [Competent Cell Protocols](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/microbial-cell-culture/competent-cells) - [Calcium Phosphate Transfection Kit Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/calcium-phosphate-transfection-kit) - [Escort™ III Transfection Reagent Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/escort-iii-transfection-reagent-protocol) - [Universal Transfection Reagent Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/universal-transfection-reagent-general) - [Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/xtg-cell-protocols) - [Co-transfection of Plasmid DNA](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/xtg-cotransfect-pdna) - [X-tremeGENE™ 9 DNA Transfection Reagent Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/xtg9-general-protocol) - [X-tremeGENE™ HP DNA Transfection Reagent Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/xtghp-general-protocol) Top __Sign In To Continue__ To continue reading please sign in or create an account. 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