細胞アプリケーション

オンラインプロトコール

プロトコール

> Liberase DL
> Liberase TL
> Liberase DH
> Liberase TM
> Liberase TH
> Liberase MNP-S
> Liberase MTF/CT
> Collagenase A
> Collagenase B
> Collagenase D
> Collagenase H
> Collagenase P
> Collagenase/Dispase®
> Dispase® I
> Dispase® II

Liberase DL

Mouse lung / >Isolation of lung cells
>Isolation of lung cells
  spleen / 脾臓 >Isolation of dendritic cells
  pancreas / 膵臓 >Isolation of mouse islets cells

Liberase TL

Human pancreas / 膵臓 >Isolation of pancreatic β-cells
  tumor cells / 腫瘍細胞 >Isolation of melanoma tumor stem cells (MTSC)
Mouse lymph node / リンパ節 >Single-cell suspensions generated by digesting lymph node
>Isolation of dendritic cell from lymph nodes
  spleen / 脾臓 >Isolation of splenic dendritic cells (DCs)
  skin / 皮膚 >Isolation of GFP+ Mouse Skin Cells
  lung / >Cell suspension from lung tissue

Liberase DH

Human skin / 皮膚 >Generation of skin-derived precursor (SkP) cells from human Skin
Mouse cardiac cells / 心臓細胞 >Isolation of Cardiomyocytes
>Heart Tissue/Cardiomyocytes Isolation
  osteoblast cell line / 骨芽細胞株 >Extraction of the extra cellular matrix (ECM) Constituents

Liberase TM

Human adipose tissue / 脂肪組織 >>Isolation of adipose tissue-derived stem cells from human subcutaneous fat tissue
>Isolation of adipocytes from human adipose tissue
  tumor cells / 腫瘍細胞 >Dissociation of human brain tumor tissue
  cardiac cells / 心臓細胞 >Cell detachment of induced pluripotent stem cell (iPSC) derived cardiomyocytes
Mouse liver / 肝臓 >Isolation of parenchymal hepatic cells from mouse fetal liver
>Isolation of primary hepatocytes from mouse
>Mouse hepatocyte isolation
  lymph node / リンパ節 >Digestion of mediastinal lymph node (MLN) for pulmonary dendritic cell migration studies in mice
  spleen / 脾臓 >Isolation of Gastric lamina propria leukocyte (LPL)
Zebrafish embryonic stem cells / 胚性幹細胞/ES細胞 >Embryonic stem cells from zebrafish embryos
  tumor cells / 腫瘍細胞 >Isolation of transplanted tumor cells from zebra fish

Liberase TH

Mouse cardiac cells / 心臓細胞 >Isolation of cardiac myocytes
  thymus tissue / 胸腺組織 >Digestion of adult thymus tissue
  tumor cells / 腫瘍細胞 >Isolation of melanoma tumor stem cells (MTSC)

Liberase MNP-S

Human adipose tissue / 脂肪組織 >Isolation of human adult stem cells from fat tissue using Cytori´s Celution® System

Liberase MTF/CT

Human pancreas / 膵臓 >Islet cell isolation
>Islet cell isolation

Collagenase A

Human endothelial cells / 内皮細胞 >Isolation of endothelial cells (HUVECs) from umbilical cord
>
Isolation of endothelial cells from retroperitoneal adipose tissue or nasal mucosa (human)
  neural tissue / 神経組織 >Dissociation of of nerve explants for establishing purified human Schwann cells
  decidua / 脱落膜 >Isolation of human decidual tissue from first trimester legal abortion material
Xenopus oocytes / 卵母細胞 >Dissociation of Xenopus laevis oocytes
>Defolliculation of Xenopus laevis oocytes by collagenase treatment
>Removal of follicular tissue from Xenopus laevis oocytes
>Preparation of Xenopus laevis oocytes
Mouse embryoid body / 胚様体 >Fluorescence-activated cell sorting (FACS) of specific cell lineages in mouse embryoid bodies

Collagenase B

Human embryoid body / 胚様体 >96-Well embryoid body differentiation of human embryonic stem cells
>Dissociation of human embryoid bodies for differentiation toward the cardiomyocyte lineage
>Dissociation of human embryoid bodies for vascular differentiation
  embryonic stem cells / 胚性幹細胞/ES細胞 >Fluorescence-activated cell sorting (FACS) of human embryonic stem cells
  mesenchymal stem cells / 間葉系幹細胞 >Isolation of human umbilical cord stroma-derived mesenchymal stem cells (hUCS-MSCs)
Mouse embryoid body / 胚様体 >Dissociation of mouse embryoid bodies for cardiac, skeletal muscle, and vascular smooth muscle cell differentiation
  embryonic stem cells / 胚性幹細胞/ES細胞 >Isolation of single beating cardiomyocytes from mouse embryonic stem cells
  muscle stem cells / 筋幹細胞 >Mouse muscle stem cell isolation by fluorescence activated cell sorting (FACS) Cytometry
  cardiac cells / 心臓細胞 >Isolation of cardiac progenitor cells by enzymatic digestion of murine hearts

Collagenase D

Human cartilage / 軟骨 >Isolation of fibroblasts from human connective tissue
>Isolation of chondrocytes from human cartilage specimens
  muscle tissue / 筋組織 >Dissociation of primary human fetal skeletal muscle tissue for isolation of fetal myoblasts
>Isolation of myoblasts from human muscle tissue
Mouse spleen (lymph node; thymus) / 脾臓(リンパ節; 胸腺) >Isolation of dendritic cells (DCs) from mouse spleen
>Isolation of dendritic cells (DCs) from mouse spleen and lymph nodes
>Isolation of dendritic cells (DCs) from mouse spleen, thymus, and lymph nodes
  colon / 結腸 >Isolation of lamina propria mononuclear cells (LPMCs) from mouse colons
  muscle tissue / 筋組織 >Isolation of primary myoblasts from mouse muscles
  olfactory epithelium / 嗅上皮 >Dissection of the Mouse Olfactory Epithelium (2002)
>Dissection of the Mouse Olfactory Epithelium (2008)
  bone marrow / 骨髄 >Isolation of murine macrophages from bone marrow and peritoneal cavity
Rat liver / 肝臓 >Isolation of hepatocytes from young and old rats
  neuronal tissue / 神経組織(ニューロン) >Neuron dissociation for expressing signaling proteins in adult rat sympathetic neurons by microinjection

Collagenase H

Human skin / 皮膚 >Extraction of fibroblasts from human skin
Rat liver / 肝臓 >Isolation of hepatic stem cells from rat livers

Collagenase P

Human cartilage / 軟骨 >Isolation of isolated human chondrocytes from articular and costal cartilage
  embryonic stem cells / 胚性幹細胞/ES細胞 >In vitro derivation of chondrogenic cells from human embryonic stem cells
Mouse pancreas / 膵臓 >Isolation of pancreatic islet cells from nonobese diabetic (NOD) mice
>Isolation of islets from mouse pancreas

Collagenase/Dispase ®

Human liver / 肝臓 >CD34+ Cell isolation from human fetal liver tissue
Mouse muscle tissue / 筋組織 >Isolation of mouse satellite cells by enzymatic dissociation of skeletal muscles
  embryonic stem cells / 胚性幹細胞/ES細胞 >Generation of osteoblasts and chondrocytes from embryonic stem cells / dissociation of embryoid bodies

Dispase® I

Human prostate / 前立腺 >Procurement of prostate tissue for establishment of human primary prostate cell cultures
  neural tissue / 神経組織 >Dissociation of of nerve explants for establishing purified human Schwann cells
Mouse olfactory epithelium / 嗅上皮 >Preparation of a single-cell suspension fom mouse olfactory epithelium (2008)
>Preparation of a single-cell suspension fom mouse olfactory epithelium (2002)
  intestine / 腸管 >Isolation of intestinal cells from fetal mouse or rat intestine
  submandibular gland / 顎下腺 >Dissection of mouse embryonic submandibular gland for preparation of epithelial cells
  tumor cells / 腫瘍細胞 >Preparation of mouse tumor cells (e.g. leukemia) for clonogenic assays
Chick neuronal tissue / 神経組織(ニューロン) >Dissection of hindbrain from chick embryos
Various embryonic tissue / 胚組織 >Dissection of mouse, rat, and chick embryonic tissues intended for embedding in collagen gels

Dispase® II

Human skin / 皮膚 >Keratinocyte isolation from human skin intended for establishment of keratinocyte stem cells
>Extraction of keratinocytes from human skin
>Isolation of human keratinocytes from foreskin tissue samples
>Isolation of keratinocytes from neonatal foreskin for anlysis by flow cytometry
>Isolation of human keratinocyte stem cells from skin for FACS enrichment
>Isolation of human dermal stem cells that differentiate into epidermal melanocytes
>Isolation of normal human epidermal melanocytes from skin specimens
>Isolation and cultivation of normal human epidermal melanocytes
>Human fibroblasts from skin biopsies for generation of induced pluripotent stem cells
>Isolation of dendritic cells (DCs) based on enzymatic digestion of skin tissue from mouse and man
>Recovery of intact human epidermis from rat trachea after transplantation
  amnion / 羊膜 >Human amniotic and placental mesenchymal stem cell isolation
>Isolation human amnion membrane-derived primary cells for generation of induced pluripotent stem cells
  adipose tissue / 脂肪組織 >Isolation of adipose-derived stem cells from adult human adipose tissue
  mammary tissue / 乳腺組織 >Enzymatic dissociation and culture of normal human mammary tissue to detect progenitor activity
  hematopoietic cells / 造血細胞 >Preparation of human hematopoietic progenitor cells for immunoselection and FACS
Mouse skin / 皮膚 >Processing of adult murine skin (mouse tails) for isolation of basal keratinocytes
>Treatment of mouse skin samples for performing Chromatin Immunoprecipitation in keratinocytes
>Isolation of dendritic cells (DCs) based on enzymatic digestion of skin tissue from mouse and man
  muscle tissues / 筋組織 >Isolation of muscle stem cells from mouse by fluorescence activated cell sorting cytometry (FACS)
>Isolation of primary myoblasts from mouse muscles
  embryoid body / 胚様体 >Disaggregation of mouse embryoid bodies into a single cell suspension
  ES cell lumps / ES細胞塊 >Digestion of mouse embryonic stem cells for generation of ES cell clumps
>Mouse embryonic stem cell differentiation and generation of ES cell clumps
  cardiac cells / 心臓細胞 >Isolation of cardiac progenitor cells by enzymatic digestion of murine hearts
Avian embryonic tissue / 胚組織 >Dissection of avian embryonic tissue for preparation of neural crest
>Cultures of unsegmented mesoderm from avian or mouse embryos
Rat neural tissue / 神経組織 >Isolation of neural precursor cells from rat fetal tissue
>Isolation of neural precursor cells from rodent tissue
Xenopus oocytes / 卵母細胞 >Defolliculation of Xenopus laevis oocytes
Aplysia neuronal tissue / 神経組織(ニューロン) >Isolation of neurons from Aplysia ganglion
  cornea / 角膜 >Enzymatic digestion of limbus corneal epithelial layer
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