核酸の増幅 (PCR/RT-PCR)

PCR試薬とセレクトガイド

> ルーチンおよびホットスタート用途
> 特殊な用途

ルーチンおよびホットスタート用途

PCR Application Routine PCR Fast PCR Hot Start Multiplex/Sequencing
Your Enzyme
of Choice:
Taq DNA Polymerase AptaTaq Fast
DNA Polymerase
FastStart Taq
DNA Polymerase
FastStart High
Fidelity PCR System
Experience Consistency Speed Specificity Accuracy
Amplicon Size up to 3 kb up to 500 bp up to 3 kb up to 5 kb
Specificity ■ ■ □ □ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■
Sensitivity ■ ■ □ □ ■ ■ □ □ ■ ■ ■ ■ ■ ■ ■ ■
Robustness ■ ■ □ □ ■ ■ ■ ■ ■ ■ ■ □ ■ ■ ■ □
Accuracy vs. Taq 1x 1x 1x 4x
Carryover Prevention yes yes yes yes
Units/50 μl 1.25 2/20 μl 2 2.5
Molecular Cloning TA cloning TA cloning TA cloning TA cloning
Enzyme
dNTPack (dNTP付属)  
Master Mix  

特殊な用途

PCR Application Wide Variety/Easy Access High Fidelity Difficult Templates Long Range
Your Enzyme of Choice: Expand High Fidelity
PCR System
Pwo SuperYield DNA Polymerase GC-RICH PCR System Expand Long Range
Experience Robustness Fidelity Reliability Versatility
Amplicon Size up to 5 kb up to 3 kb up to 5 kb 5 to 25 kb**
Specificity ■ ■ ■ □ ■ ■ □ □ ■ ■ ■ □ ■ ■ ■ □
Sensitivity ■ ■ ■ □ ■ ■ □ □ ■ ■ ■ □ ■ ■ □ □
Robustness ■ ■ ■ ■ ■ ■ □ □ ■ ■ ■ □ ■ ■ ■ □
Accuracy vs. Taq 3x 18x 3x 3x
Carryover Prevention no* no no no
Units/50 μl 2.6 2.5 2 3.5
Molecular Cloning TA cloning Blunt end cloning TA cloning preferred over blunt end cloning TA cloning
Enzyme  
dNTPack (dNTP付属)
Master Mix    

* Requiring carryover prevention and 6x higher fidelity? Choose Expand High FidelityPLUS PCR System.
** For amplicon sizes of 20-35 kb choose Expand 20 kbPLUS PCR System.