Membrane Preparations and Reagents for Transporter Assays

Ready-to-use membrane preparations and reagents from SOLVO Biotechnology® are powerful tools for studying drug transporters and are now available at Sigma-Aldrich. SOLVO’s vesicular transport and ATPase models provide superior transporter activity, higher dynamic range, and more physiologically-relevant lipid composition allowing optimal investigations of drug-transporter interactions.

Vesicular Transporter Assays
Membrane Preparations for Vesicular Transport Assays are suitable for general drug-transporter interaction studies. Both substrate and inhibitor interactions can be assessed using vesicles over-expressing key transporters.

Negative control membranes are also available.
ATPase Transporter Assays
The ATPase assay is an in vitro membrane assay designed to indicate the nature of the interaction between a compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated.

Negative control membranes are also available.
HEK293-based Membrane Preparations for Transporter Assays
Product No. Description
SBVT14 SB-MRP2-HEK293
SBVT15 SB-MRP3-HEK293
SBVT16 SB-MDR1-HEK293 recombinant, expressed in HEK 293 cells
SBVT17 SB-BCRP-HEK293
SBVT18 SB-BSEP-HEK293
SBVT19 SB-mouseMdr1a-HEK293 recombinant, expressed in HEK 293 cells
SBVT20 SB-ratMdr1b-Sf9-HAM recombinant, expressed in baculovirus infected Sf9 cells
SBVT21 SB-ratBsep-HEK293 recombinant, expressed in HEK 293 cells
SBVT27 SB-cyBSEP-HEK293 
SBVT28 SB-dogBSEP-HEK293 
SBVT29 SB-cyMDR1-HEK293 
SBVT30 SB-cyBcrp-HEK293 
 
Negative Control Membrane
Product No. Description
SBCT01 SB-K-CTRL recombinant, expressed in human cells (proprietary human “K” cells)
SBCT02 SB M CTRL recombinant, expressed in human cells (expressed in proprietary human “M” cells)
SBCT03 SB beta gal Sf9 CTRL recombinant, expressed in baculovirus infected Sf9 cells
SBCT04 SB-beta-gal-Hi5-CTRL recombinant, expressed in Baculovirus infected High-5 cells
SBCT06 SB-HEK293-Mock-CTRL Negative control membrane for HEK293 transporter membranes
SBCT10 SB-beta-gal-Sf9-HAM-CTRL recombinant, expressed in baculovirus infected Sf9 cells
 
PREDIVEZ™ Reagent Kits
Product No. Description
SBPVR1 SB PREDIVEZ™ Reagent Kit for MDR1/P-gp
SBPVR2 SB PREDIVEZ™ Reagent Kit for MRP1
SBPVR3 SB PREDIVEZ™ Reagent Kit for MRP2, MRP3, and MRP5
SBPVR4 SB PREDIVEZ™ Reagent Kit for BCRP
 
Kits for Transporter Assays
Product No. Description
SBPE01 SB-MDR1-Hi5-PREDEASY-ATPase kit membrane based kit for Human MDR1 ATPase Transport Assays
SBPE02 MRP1 human membrane based kit for Human MRP1 ATPase Transport Assays
SBPE03 SB-MRP2-Sf9-PREDEASY-ATPase kit membrane based kit for Human MRP2 ATPase Transport Assays
SBPE04 MRP3 human membrane based kit for Human MRP3 ATPase Transport Assays
SBPE05 SB-BCRP-M-PREDEASY-ATPase kit membrane based kit for Human BCRP ATPase Transport Assays
SBPE06 SB-ratMdr1b-Sf9-PREDEASY-ATPase kit membrane based kit for Rat Mdr1b ATPase Transport Assays
SBPE12 SB-BCRP-HAM-PREDEASY-ATPase
SBPE13 SB-mouseBsep-Sf9-HAM-PREDEASY-ATPase kit
 
Transporter Assay Reagents
Product No. Description
SBNMQ NMQ/N-methyl-quinidine - MDR1/P-gp Substrate
SBPSC PSC833 - Specific MDR1/P-gp Inhibitor