Membrane Preparations and Reagents for Transporter Assays

Ready-to-use membrane preparations and reagents from SOLVO Biotechnology® are powerful tools for studying drug transporters and are now available at Sigma-Aldrich. SOLVO’s vesicular transport and ATPase models provide superior transporter activity, higher dynamic range, and more physiologically-relevant lipid composition allowing optimal investigations of drug-transporter interactions.

Vesicular Transporter Assays
Membrane Preparations for Vesicular Transport Assays are suitable for general drug-transporter interaction studies. Both substrate and inhibitor interactions can be assessed using vesicles over-expressing key transporters.

Negative control membranes are also available.
ATPase Transporter Assays
The ATPase assay is an in vitro membrane assay designed to indicate the nature of the interaction between a compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated.

Negative control membranes are also available.
HEK293-based Membrane Preparations for Transporter Assays
Product No. Description
SBVT14 SB-MRP2-HEK293
SBVT15 SB-MRP3-HEK293
SBVT16 SB-MDR1-HEK293 recombinant, expressed in HEK 293 cells
SBVT17 SB-BCRP-HEK293
SBVT18 SB-BSEP-HEK293
SBVT19 SB-mouseMdr1a-HEK293 recombinant, expressed in HEK 293 cells
SBVT20 SB-ratMdr1b-Sf9-HAM recombinant, expressed in baculovirus infected Sf9 cells
SBVT21 SB-ratBsep-HEK293 recombinant, expressed in HEK 293 cells
SBVT27 SB-cyBSEP-HEK293 
SBVT28 SB-dogBSEP-HEK293 
 
Membrane Preparations for Transporter Assays
Product No. Description
SBVT01 SB-MDR1-K recombinant, expressed in human cells (proprietary human “K” cells)
SBVT02 SB-BSEP-Sf9 membrane preparation for Vesicular Transport Assays, recombinant, expressed in baculovirus infected Sf9 cells
SBVT03 SB-BSEP-Hi5 Membrane preparation for Vesicular Transport Assays, recombinant, expressed in Baculovirusinfected High-5 cells
SBVT04 SB-MRP1-Sf9 membrane preparation for Vesicular Transport Assays, recombinant, expressed in baculovirus infected Sf9 cells
SBVT05 SB-MRP2-Sf9 membrane preparation for Vesicular Transport Assays, recombinant, expressed in baculovirus infected Sf9 cells
SBVT06 SB-MRP3-Sf9 membrane preparation for Vesicular Transport Assays, recombinant, expressed in baculovirus infected Sf9 cells
SBVT07 SB-MRP4-HEK293 MRP4 human membrane vesicles
SBVT08 SB-MRP5-HEK293 MRP5 human membrane vesicles
SBVT09 SB-BCRP-M recombinant, expressed in human cells (expressed in proprietary human “M” cells)
SBVT10 BCRP human membrane preparation for Vesicular Transport Assays, recombinant, expressed in human amnion-derived mesenchymal (HAM) cells
SBVT12 SB-mouseBsep-Sf9 membrane preparation for Vesicular Transport Assays, recombinant, expressed in baculovirus infected Sf9 cells
SBVT13 SB-ratMrp2-HEK293 MRP2 rat vesicles
SBVT29 SB-cyMDR1-HEK293 
SBVT30 SB-cyBcrp-HEK293 
 
Negative Control Membrane
Product No. Description
SBCT01 SB-K-CTRL recombinant, expressed in human cells (proprietary human “K” cells)
SBCT02 SB M CTRL recombinant, expressed in human cells (expressed in proprietary human “M” cells)
SBCT03 SB beta gal Sf9 CTRL recombinant, expressed in baculovirus infected Sf9 cells
SBCT04 SB-beta-gal-Hi5-CTRL recombinant, expressed in Baculovirus infected High-5 cells
SBCT06 SB-HEK293-Mock-CTRL Negative control membrane for HEK293 transporter membranes
SBCT10 SB-beta-gal-Sf9-HAM-CTRL recombinant, expressed in baculovirus infected Sf9 cells
 
PREDIVEZ™ Reagent Kits
Product No. Description
SBPVR1 SB PREDIVEZ™ Reagent Kit for MDR1/P-gp
SBPVR2 SB PREDIVEZ™ Reagent Kit for MRP1
SBPVR3 SB PREDIVEZ™ Reagent Kit for MRP2, MRP3, and MRP5
SBPVR4 SB PREDIVEZ™ Reagent Kit for BCRP
 
Kits for Transporter Assays
Product No. Description
SBPE01 SB-MDR1-Hi5-PREDEASY-ATPase kit membrane based kit for Human MDR1 ATPase Transport Assays
SBPE02 MRP1 human membrane based kit for Human MRP1 ATPase Transport Assays
SBPE03 SB-MRP2-Sf9-PREDEASY-ATPase kit membrane based kit for Human MRP2 ATPase Transport Assays
SBPE04 MRP3 human membrane based kit for Human MRP3 ATPase Transport Assays
SBPE05 SB-BCRP-M-PREDEASY-ATPase kit membrane based kit for Human BCRP ATPase Transport Assays
SBPE06 SB-ratMdr1b-Sf9-PREDEASY-ATPase kit membrane based kit for Rat Mdr1b ATPase Transport Assays
SBPE12 SB-BCRP-HAM-PREDEASY-ATPase
SBPE13 SB-mouseBsep-Sf9-HAM-PREDEASY-ATPase kit
 
Membrane Preparations for ATPase Assays
Product No. Description
SBAT06 BCRP human membrane preparation for ATPase Assays, recombinant, expressed in baculovirus infected Sf9 cells
 
Transporter Assay Reagents
Product No. Description
SBNMQ NMQ/N-methyl-quinidine - MDR1/P-gp Substrate
SBPSC PSC833 - Specific MDR1/P-gp Inhibitor