Avanti® Mini-Extruder

Avanti® Mini-Extruder

 

The Avanti® Mini Extruder allows researchers and scientists to prepare large, unilamellar vesicles by extrusion in an efficient manner.

Convenient

  • Prepare large, unilamellar vesicles (LUVs) by extrusion inan easy, rapid, and efficient manner.
  • Design allows for the rapid cleaning of all wetted parts,
    reducing downtime between production of vesicles from different lipid species.
  • Generate uniform populations of unilamellar liposomes without the use of solvents or detergents.
  • Control vesicle size via polycarbonate membrane selection.

Complete set

  • Mini extruder
  • Heating block
  • 2 O-rings
  • 2 Gas-tight syringes
  • 100 polycarbonate membranes
  • 100 filter supports

Durable and Affordable

  • Constructed of stainless steel and PTFE.
  • Guaranteed to give years of trouble-free service.
  • Replacement parts are available from one source.
  • A fraction of the price of a larger model

Flexible

The optional heating block allows the extrusion of vesicles at elevated temperatures, which is critical for the successful production of vesicles from phospholipids with a phase transition temperature above room temperature.

  • Generate unilamellar liposomes ranging from 30 nm–1 μm when assembled with appropriate polycarbonate membrane.
  • Useful for a wide variety of lipid compositions.
  • Interchangeable with 250 μL and 1 mL syringes.

Reliable

The particle size distribution of unilamellar vesicles prepared by extrusion is a function of the number of passes through the extruder membrane.

Hydrated lipid solutions will initially form large, multi-lamellar vesicles. After the initial pass through a membrane, the particle size distribution will tend towards bimodal. After sufficient passes through the membrane, a unimodal, normal distribution is obtained. A minimum of eleven passes through the extruder membrane is recommended for most lipids.

Placing the heating block assembly on a hot plate achieves rapid temperature control for lipids that have transition temperatures above room temperature

 

 Before you start with your new Avanti® Mini Extruder

Before assembling the extruder for the first time, thoroughly clean all parts (except for the Polycarbonate Membranes and the Filter Supports) with a mild detergent solution, followed by rinsing with hot tap water and deionized or distilled water. Allow all parts to dry before assembling apparatus. The original Mini-Extruder design contains flat washers, and a bearing washer, that are not stainless steel and will rust if not thoroughly dried.

The extruder apparatus must be fully assembled before inserting in the heating block, otherwise it will be damaged. The Syringes should fit tight into the Extruder. Polycarbonate Membranes and Filter Supports are intended to be used for a single liposome preparation and should not be reused.

Autoclaving the Teflon inserts is not recommended as slight distortion may occur (~0.002mm). The use of ethylene oxide or gamma irradiation is suggested as an alternative. The syringes should be sterilized with ethanol.

NOTE: To reduce the dead volume, pre-wet the extruder parts by passing a syringe full of buffer through the extruder; discard the buffer. New syringes may have tight fitting parts; to facilitate extrusion, pre-wet syringe barrel and plunger with buffer prior to inserting plunger into barrel.

Do not allow the syringes to come into contact with any solvents other than DI water or alcohol. Some organic solvents will interact with the glue holding the threaded insert onto the tip of the syringe possibly weakening the bond between the barrel and the insert. The syringes must not be soaked in any solvent for the same reason.


 Learning & Resources