Dextranase for Dextran Hydrolysis

Dextran
It is composed of approximately 95% α-D-(1-6) linkages. The remaining a(1-3) linkages account for the branching of dextran.1,2,3 Conflicting data on the branch lengths implies that the average branch length is less than three glucose units.4,5 However, other methods indicate branches of greater than 50 glucose units exist.6,7 Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million.8,9,10 Lower MW dextrans will exhibit slightly less branching4 and have a more narrow range of MW distribution.11 Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry.7,12,13 Dextrans with MW of 2,000 to 10,000 dextran molecules exhibit the properties of an expandable coil.12 At MWs below 2,000 dextran is more rod-like.14
Dextranase
EC# 3.2.1.11
Synonym: 1,6-α-D-glucan 6-glucanohydrolase
Dextranase catalyzes the endohydrolysis of 1,6-α-D-glucosidic linkages in dextran.15
References
1. Rankin, J.C. and Jeanes, A., J. Am. Chem. Soc., 76, 4435 (1954).
2. Dimler, R.J. et al., J. Am. Chem. Soc., 77, 6568 (1955).
3. Van Cleve, J.W. et al., J. Am. Chem. Soc., 78, 4435 (1956).
4. Lindberg, B. and Svensson, S., Acta. Chem. Scand., 22, 1907 (1968).
5. Larm, O. et al., Carbohydr. Res. 20, 39 (1971).
6. Bovey, F.A., J. Polym. Sci., 35, 167 (1959).
7. Senti, R.F. et al., J. Polym. Sci., 17, 527 (1955).
8. Arond, L.H. and Frank, H.P., J. Phys. Chem., 58, 953 (1954).
9. Elias, H.G., Makromol. Chem., 33, 166 (1959).
10. Antonini, E. et al., Biopolymers, 2, 27 (1964).
11. Supplier′s data.
12. Granath, K.A., J. Colloid Sci., 13, 308 (1958).
13. Wales, M. at al., J. Polym. Sci., 66, 101 (1979).
14. Gekko, K., Am. Chem. Soc. Symposium Series, 150, 415 (1981).
15. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/11.html)

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D0443 Dextranase from Chaetomium erraticum
D8144 Dextranase from Penicillium sp. lyophilized powder, 400-800 units/mg protein
D5884 Dextranase from Penicillium sp. lyophilized powder, 10-25 units/mg solid
D4668 Dextranase from Penicillium sp. lyophilized powder, 100-250 units/mg protein