Heparinases for Heparin and Heparan Sulfate Hydrolysis

Heparin and Heparan Sulfate
These glycosaminoglycans (GAGs) are complex heterogeneous mixtures of repeating disaccharide units consisting of a uronic acid (D-glucuronic or L-iduronic acid) and D-glucosamine or N-acetyl-D-glucosamine. Various degrees of sulfation occur (at O and/or N) on each monosacchiride unit, ranging from zero to tri-sulfation. In general, heparan is less sulfated than heparin.

Analysis of commercial products that contain heparin has taken on increasing importance because of concerns about heparin quality and integrity in commercial items such as pharmaceutical products.
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