SILuProt Protein Standards for Quantitative Mass Spectrometry

SILuProt products are a novel collection of stable isotope-labeled (SIL, or “heavy labeled”) full length proteins designed to be used as mass spectrometry internal standards for quantitative proteomics. The ideal method of optimizing reproducibility and accuracy in protein quantification by mass spectrometry is to add a full length SIL recombinant protein, equivalent to the native target protein, to the sample at the initial stage of sample preparation. Introduction of an internal standard at the beginning of the MS workflow minimizes variations in quantification due to protein extraction, fractionation, enrichment, proteolysis and analysis. Sigma-Aldrich also offers all of the SILuProt products in their unlabeled form, SILuLite

Poster for SILuProt products: Accurate internal standards in Quantitative Mass Spectrometry

Published use of recombinant SILuProt as internal standards:Absolute Protein Quantification by Mass Spectrometry: Not as Simple as Advertised

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MSST0049 SILuProt AKT1,RAC-alpha serine/threonine-protein kinase human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C- and 15N-labeled
MSST0051 SILuProt AKT2, RAC-beta serine/threonine-protein kinase human recombinant, expressed in HEK 293 cells, 13C and 15N-labeled, SIL MS Protein Standard
MSST0053 SILuProt AKT3, RAC-gamma serine/threonine-protein kinase human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0011 SILuProt ALB Albumin human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0013 SILuProt AMBP Alpha-1 Microglycoprotein human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0001 SILuProt APOA1, Apolipoprotein A-1 human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C- and 15N-labeled
MSST0029 SILuProt APOA2 Apolipoprotein A-II human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0061 SILuProt APOD, Apolipoprotein D human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0015 SILuProt B2M beta-2-microglobulin human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C- and 15N-labeled
MSST0035 SILuProt CCL2 C-C motif chemokine 2 human SIL MS Protein Standard, 13C and 15N-labeled
MSST0007 SILuProt CLU Clusterin human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0033 SILuProt COL1A1 N-terminal propeptide human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0021 SILuProt CRP C-reactive protein human recombinant, expressed in HEK 293 cells, 13C and 15N-labeled
MSST0059 SILuProt CST3, Cystatin C human recombinant, expressed in E. coli, SIL MS Protein Standard, 15N-labeled
MSST0041 SILuProt HAVCR1 Hepatitis A virus cellular receptor 1 human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0039 SILuProt IFNG Interferon Gamma human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0037 SILuProt IGFBP7 Insulin-like growth factor-binding protein 7 human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0017 SILuProt IL6 Interleukin 6 human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C- and 15N-labeled
MSST0057 SILuProt IL8, Interleukin-8 human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0064 SILuProt Insulin, human recombinant, expressed in Pichia pastoris, SIL MS Protein Standard, 15N -labeled New
MSST0009 SILuProt MAPK1 Mitogen Activated Protein Kinase 1 human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N -labeled
MSST0019 SILuProt MAPK3 Mitogen activated protein kinase 3 human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0031 SILuProt MAPT Microtubule-associated protein tau-441 human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0003 SILuProt PTX3 Pentraxin-related protein PTX3 human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C- and 15N-labeled
MSST0047 SILuProt SOST, Sclerostin human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0043 SILuProt TIMP1, Metalloproteinase inhibitor 1 human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0045 SILuProt TIMP2, Metalloproteinase inhibitor 2 human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled
MSST0005 SILuProt VEGFA Vascular endothelial growth factor A human recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C- and 15N-labeled