Organic Synthesis

Immobilization of enzymes on solid supports, for application to organic synthesis, offers such conveniences as facile separation of products from the supported enzyme matrix. Potential large-scale applications of immobilized enzymes include production of biorenewables. The classic legacy Sigma-Aldrich enzymes portfolio includes select immobilized enzymes for your research needs into such fields as biocatalysis.

1. Brena, B.M., and Batista-Viera, F., “Immobilization of Enzymes: A Literature Survey”, in Immobilization of Enzymes and Cells (Guisán, J.M., editor). Methods in Biotechnology (ISBN 978-1-59745-053-9), pp. 15-30 (2006).
2. Franssen, M.C.R., et al., “Immobilised enzymes in biorenewables production”. Chem. Soc. Rev., 42, 6491-6533 (2013). Publication
3. Mohamad, N.R., et al., “An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes”. Biotechnol. Biotechnol. Equip., 29(2), 205-220 (2015).. Publication

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A3010 Acylase I from porcine kidney Grade I, lyophilized powder, ≥1500 units/mg protein
A2714 Aldolase from rabbit muscle lyophilized powder, ≥8.0 units/mg protein
A8811 Aldolase from rabbit muscle ammonium sulfate suspension, 10-20 units/mg protein
C9830 Chitosanase from Streptomyces griseus lyophilized powder, >50 units/mg protein (Bradford)
45299 Epoxide Hydrolase from Rhodococcus rhodochrous lyophilized powder, beige, ≥0.2 U/g
46051 Esterase from Bacillus stearothermophilus ≥0.2 U/mg
E3019 Esterase from porcine liver lyophilized powder, ≥15 units/mg solid
E2884 Esterase from porcine liver ammonium sulfate suspension, ≥150 units/mg protein (biuret)
46058 Esterase from porcine liver lyophilized, powder, slightly beige, ≥50 U/mg
E0887 Esterase from rabbit liver lyophilized powder, ≥30 units/mg protein
79208 Esterase from Rhizopus oryzae powder, light beige, ≥20 U/g
G1288 β-(1→3,4,6)-Galactosidase, positionally specific recombinant, expressed in E. coli, buffered aqueous solution
G0413 β(1→4)-Galactosidase, positionally specific from Streptococcus pneumoniae recombinant, expressed in E. coli, buffered aqueous solution
G7163 α-Galactosidase, positionally specific from Escherichia coli recombinant, expressed in E. coli, buffered aqueous solution
62301 Lipase from Aspergillus niger powder (fine), ~200 U/g
62285 Lipase from Aspergillus oryzae lyophilized, powder, white, ~50 U/mg
02569 Lipase from Candida antarctica lyophilized, powder, beige, ~0.3 U/mg
L1754 Lipase from Candida rugosa Type VII, ≥700 unit/mg solid
L8525 Lipase from Candida rugosa lyophilized powder, ≥40,000 units/mg protein
62316 Lipase from Candida rugosa powder, yellow-brown, ≥2 U/mg
62302 Lipase from Candida rugosa lyophilized, powder (fine), 15-25 U/mg
L8906 Lipase from Mucor javanicus lyophilized powder, ≥300 units/mg solid (using olive oil)
62298 Lipase from Mucor miehei powder, slightly brown, ~1 U/mg
L9031 Lipase from Mucor miehei lyophilized powder, ≥4,000 units/mg solid (using olive oil)
L3126 Lipase from porcine pancreas Type II, 100-650 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
L0382 Lipase from porcine pancreas Type VI-S, ≥20,000 units/mg protein, lyophilized powder
62309 Lipase from Pseudomonas cepacia powder, light beige, ≥30 U/mg
L9518 Lipase from Pseudomonas sp. Type XIII, lyophilized powder, ≥15 units/mg solid
62310 Lipase from Rhizopus niveus powder (fine), ≥1.5 U/mg
62305 Lipase from Rhizopus oryzae powder (fine), ~10 U/mg
L3001 Lipase from wheat germ Type I, lyophilized powder, 5-15 units/mg solid
62306 Lipase from wheat germ ~0.1 U/mg
62287 Lipase A Candida antarctica, recombinant from Aspergillus oryzae powder, beige, ~2 U/mg
62288 Lipase B Candida antarctica, recombinant from Aspergillus oryzae powder, beige, ~9 U/mg
62335 Lipoprotein Lipase from Pseudomonas sp. lyophilized, powder, ≥1200 U/mg
P8913 Phospholipase A2 from bovine pancreas lyophilized powder, ≥20 units/mg protein
P9279 Phospholipase A2 from honey bee venom (Apis mellifera) salt-free, lyophilized powder, 600-2400 units/mg protein
P6534 Phospholipase A2 from porcine pancreas ammonium sulfate suspension, ≥600 units/mg protein
P4039 Phospholipase C from Clostridium perfringens (C. welchii) Type XIV, lyophilized powder, ≥150 units/mg protein Green Alternative
P8398 Phospholipase D from cabbage Type IV, lyophilized powder, ≥100 units/mg solid
P4912 Phospholipase D from Streptomyces sp. Type VII, lyophilized powder, ≥150 units/mg solid
P8804 Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)
P0515 Phospholipase D from Arachis hypogaea (peanut) Type II, lyophilized powder, ≥60 units/mg protein
77087 ω-Transaminase, Aspergillus fumigatus recombinant, expressed in E. coli, ≥0.20 U/mg
40972 ω-Transaminase, Aspergillus terreus recombinant, expressed in E. coli, ≥0.10 U/mg
T7684 Transaminase, broad-range bacterial recombinant, expressed in E. coli, lyophilized powder, ≥5 units/mg solid
93006 ω-Transaminase, Neosartorya fischeri recombinant, expressed in E. coli, ≥0.4 U/mg