Proteases for Recombinant Protein Fusion Tag Cleavage

One of the factors in choosing a recombinant protein expression platform is the option of which proteolytic fusion tag recognition site will be incorporated into the protein sequence. Fusion tags are often utilized for rapid purification and detection of recombinant proteins. Although fusion tags are vital for these particular functions, they are not considered to be permanent fixtures or domains on recombinant proteins. Hence, removal of the fusion tag is an essential requirement for highly analytical studies of recombinant protein structure and function. (Additional factors may include subsequent removal of the protease.)
Use the Protease Finder to identify individual proteases that can be used to cleave a distinct peptide bond within your specific protein or peptide sequence.

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S4576 CleanCut Protease from Staphylococcus aureus 500-1000 units/mg protein
E5144 Enterokinase from bovine intestine powder
E4906 Enterokinase from bovine intestine BioUltra, recombinant, expressed in E. coli, ≥20 units/mg protein, ≥95% (SDS-PAGE)
E0632 Enterokinase from porcine intestine ≥0.5 units/mg solid
E0885 Enterokinase from porcine intestine lyophilized powder, ≥100 units/mg protein
SRP3032 Enterokinase human recombinant, expressed in CHO cells, ≥90% (SDS-PAGE), ≥90% (HPLC), suitable for cell culture
PRKE Enterokinase Removal Kit
F9302 Factor X Activated (Xa) from bovine plasma aqueous glycerol solution
SAE0045 HRV-3C Protease. N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL
SAE0110 HRV-3C Protease, Biotin tagged Recombinant protein, 0.8-1.2 mg/mL, aqueous solution
GE27-0843-01 PreScission Protease GE Healthcare, 27-0843-01
T4455 TEV Protease
T7513 Thrombin from bovine plasma lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)
T6884 Thrombin from human plasma lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280, 18.3)
T1063 Thrombin from human plasma lyophilized powder, ≥2800 NIH units/mg protein (E1%/280, 18.3)
T9326 Thrombin human BioUltra, recombinant, expressed in HEK 293 cells, aqueous solution, ≥95% (SDS-PAGE)
RECOMT Thrombin CleanCleave Kit
GE27-0846-01 Thrombin Protease GE Healthcare, 27-0846-01